In Vivo Functional Assay of a Recombinant Aquaporin in Pichia pastoris
نویسندگان
چکیده
منابع مشابه
In vivo functional assay of a recombinant aquaporin in Pichia pastoris.
The water channel protein PvTIP3;1 (alpha-TIP) is a member of the major intrinsic protein (MIP) membrane channel family. We overexpressed this eukaryotic aquaporin in the methylotrophic yeast Pichia pastoris, and immunogold labeling of cellular cryosections showed that the protein accumulated in the plasma membrane, as well as vacuolar and other intracellular membranes. We then developed an in ...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2006
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.72.2.1507-1514.2006